RT-PCR diagnosis of COVID-19 from exhaled breath condensate: a clinical study
نویسندگان
چکیده
Abstract Current diagnostic testing for coronavirus disease 2019 (COVID-19) is based on detection of severe acute respiratory syndrome 2 (SARS-CoV-2) in nasopharyngeal swab samples by reverse transcription polymerase chain reaction (RT-PCR). However, this test associated with increased risks viral dissemination and environmental contamination shows relatively low sensitivity, attributable to technical deficiencies the sampling method. Given that COVID-19 transmitted via exhaled aerosols droplets, breath condensate (EBC) an established modality aerosols, SARS-CoV-2 EBC offers a promising approach. current knowledge load virus collected from patients remains limited inconsistent. The objective study was quantify validate feasibility as infection. were 48 using collection device, loads quantified RT-PCR targeting E gene. Changes rates relative patient characteristics days since onset statistically evaluated. Need mechanical ventilation significantly higher ( p < 0.05). oxygen administration or ventilation, less than 3 d onset, presence cough fever Among spontaneously breathing patients, attenuated exponentially over time. rate 86% at deteriorated thereafter. In mechanically ventilated high regardless onset. These results support detect within symptom
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ژورنال
عنوان ژورنال: Journal of Breath Research
سال: 2021
ISSN: ['1752-7163', '1752-7155']
DOI: https://doi.org/10.1088/1752-7163/ac0414